【摘要】 研究发现 1 0种酵母菌株均可将苯甲醛转化为PAC ,且苯甲醛对所有酵母的最小抑制作用均在 0 .2 %～0 .4 %之间。采用温度、uV、DES、LiCI以及LiCI和uV联合诱变结果 ,获得 3个转化活力较高的变异株 ,其中变异株S .CarlebergensisCPU 950 7 4 3″连续接种培养五代并进行转化和测试 ,结果转化活力均较高 ,表明变异株遗传稳定性良好。更多还原

【Abstract】 It was found that all of ten yeast strains studied in this paper could transform benzaldhyde into phenylacetycarbinol (PAC). The inhibitive concentration of benzaldhyde on all these yeast strains was between 0.2%-0.4%. With UV,DES,LiCI and UV combining with LiCI as mutagens, three mutants of the yeast with high transformation efficiency were obtained, of which the S. Carlebergensis CPU 9507-4-3” was inoculated and cultivated sequentially for 5 generation for transformation tests. The results indicated that the strains was genetically stable and could efficiently transform benzaldhyde into PAC with high transformation efficiency.更多还原