【摘要】 目的与方法 :为探Wilms瘤中 p16基因表达与肿瘤病理分型和临床分期之间的关系 ,应用免疫组织化学SP方法 ,对 5 2例Wilms瘤及 7例肿瘤周围正常肾组织 (对照 )手术标本中 p16基因的表达情况进行检测。结果 :5 2例wilms瘤中有 16例 p16基因表达阳性 ,阳性率为 30 .8% ,其中预后良好型 p16基因表达阳性率为 37.2 % (16 /4 3) ,预后差者为 0 % (0 /9) ,均明显低于正常对照 10 0 % (7/7) ,差异具有显著的统计学意义 (P <0 .0 5 )。在Ⅰ、Ⅱ、Ⅲ、Ⅳ临床期中 ,p16表达阳性率以Ⅰ期最高 ,逐步递减 ,分别为 47.6 % (10 /2 1)、37.5 % (3/8)、2 8.6 % (2 /7)、6 .3% (1/16 )。结论 :Wilms瘤中 p16基因表达与肿瘤病理分型和临床分期存在联系 ,p16基因失活似是Wilms瘤发生发展中的晚期事件。更多还原

【Abstract】 Purpose and Methods: In order to investigate the p16 gene expression and its relationship with pathological type and clinical stage, 52 Wilms tumor specimens and 7 normal renal tissues aroud tumor were stained routinely by SP immunohistochemical method. Results: Positive expression rate of p16 gene was 30.8% (16/52) in tumor specimens and significantly lower than that in control 100% (7/7) (P<0.05). The positive rates were 37.2% (16/43), 0% (0/9) in the favorable pathological type and the unfavorable pathological type, and 47.6% (10/21), 37.5% (3/8), 28.6% (2/7),6.3% (1/16) in Ⅰ,Ⅱ,Ⅲ,Ⅳ clinical stage respectively. Conclusion: The positive expression of p16 gene was correlated with pathological type and clinical stage, and p16 inactivation seems to be the later event of Wilms tumor progression.更多还原