【摘要】 用２．５％戊二醛作偶联剂，分别合成牛血清白蛋白──氨苄西林钠抗原和孵白蛋白──氨苄西林钠抗原．用牛血清白蛋白──氨苄西林钠作免疫原，经免背部多点注射，连续强化免疫五次（每次间隔１月）后，制备免抗氨苄西林钠血清。用饱和硫酸铵法纯化抗血清，获抗氨苄西林钠ＩｇＧ，经酶联免疫测定最佳效价为１：１０００．用卵白蛋白——氨苄西林用作包板抗原，最适浓度为８μｇ／Ｌ，４℃过夜，采用４１℃孵板反应温度，建立了快速测定乳中氨苄西林钠含量的酶联免疫吸附法。氨苄西林钠测定范围５～１６０μｇ／Ｌ，最小检测限为２．５μｇ／Ｌ，回收率为１００％±５％．抗血清亲和系数为１．１４×１０~７，同氨苄西林钠和青毒素Ｇ交叉反应率分别为１００％和９８％。更多还原

【Abstract】 A glutaraldehyde (2.5%) was acted as couple agent, beef seurm albume ampicillin antigen (BSA-Am) and ovalbumin-ampicillin antigen (OV-Am) were synthesized, respectively. Antiseurm against ampicillin was prepared with BSA-Am served as immunogen injected into back skin of rabbit with a number spot, antiseurm was pured by saturated amonia sulfate, With1: 1000 optimum titre (ELISA). OV-Am was acted as a coated -plate antigen, played 4℃ through night, with 8ug/L otimum concentration, determination of ampicillin content in m by ELISA was developed, with 41℃ reactive temperature. Concentration of detective ampiciliin showed 5－160μg/L, the minimum detective hat was 2.5μg/L, recovery with 100%±5 %. The antibody against ampicillin revealed affinity of 1.14×10~7, with 100K and 98% cross reaction for ampicillin and penicillin G, respectively.更多还原