【摘要】 :【目的】为探索腺病毒载体在基因治疗中的应用及其表达目的抗原的有效性。【方法】用BamHⅠ、EcoRⅠ双切质粒 pBluscriptKS+ HBs ,回收S基因片段 ,定向克隆插入质粒 pACCMVpL中相应的酶切位点 ,通过快速抽提酶切鉴定筛选重组质粒。【结果】挑取的 8个克隆经BamHⅠ、EcoRⅠ酶切鉴定 ,有 95 0bp的目的基因片段 ,证明为重组体。【结论】成功构建表达HBsAg基因的重组腺病毒载体。更多还原

【Abstract】 Objective To explore the use of adenoviral vector in gene therapy and the effectiveness of target antigen expression in it. Method S gene was released from pBluscript KS +-HBs by BamHⅠ、EcoRⅠ cutting, then inserted directionally into plasmid pACCMVpL. The recombinant vector was identified by restriction endonuclease analysis. Result All the 8 clones selected were recombinant vector, which release about 950 bp target gene fragment by BamHⅠ、EcoRⅠ digestion. Conclusion The adenoviral vector which expressed HBsAg was constructed successfully.更多还原