【摘要】 【目的】进一步证实免疫因素在脑缺血病理变化中的作用。【方法】将 6 0只局部脑缺血模型SD大鼠分为缺血 3d ,1周和 2周 3个缺血时间组 ,每组分为生理盐水对照和环孢素A治疗两个部分 ,进行TTC和HE组化染色实验 ,并对结果进行统计处理 (t检验 )。【结果】脑缺血 1周 ,环孢素处理组的组织病理学变化较生理盐水对照组明显减轻 ,脑梗塞体积和死亡的神经元数量在环孢素A处理组分别为 3 2 %和 15 8/ 0 0 6 2 5mm2 ,而生理盐水组则为 5 9%和 2 2 1/ 0 0 6 2 5mm2 ,两组之间有显著差异 (P <0 0 5 )。但在脑缺血 3d和 2周组 ,上述变化两组之间无统计学差异。【结论】环孢素A对实验大鼠的缺血性神经元具有一定保护作用 ,结果提示免疫因素的介入 ,加重了脑缺血性损伤。更多还原

【Abstract】 Objective To confirm the effect of immune factors on the pathologic change of cerebral ischemia, Methods 60 SD rats with focal cerebral iscemic model were divided into 3 day, 1 week, and 2 week post-ischemia groups, which was further divided into cyclosporin A treatment and saline control groups. The slices of the brain were stained with HE and TTC methods. Results The histopathologic change was less serious at 1 week post-ischemia in cyclosporin A group compared with the saline group, and both infarct volume and dying neurons were 3 2% and 15.8/0.0625 mm 2 respectively in cyclosporin A group, but in saline group, they were 5.9% and 22.1/0.0625 mm 2 respectively, there were apparent differences between the two groups (P<0 05), About the two indexes, at 3 day and 2 week post-ischemia, there were no apparent differences between cyclosporin A group and saline group (P>0 05). Conclusion Cyclosporin A could protect ischemic neurons in rats, and the result suggested that the intervention of immune factors may aggravate the ischemic neuronic injury.更多还原