【摘要】 【目的】观察幽门螺杆菌形态变化及生长特性 ;探讨改良布氏肉汤对幽门螺杆菌的作用。【方法】光镜下观察冻存及培养于哥伦比亚平板上的菌株在暴露于空气中不同时间段的形态变化 ;在暴露于空气后不同的时间段将菌株分别经改良布氏肉汤及哥伦比亚平板进行传代培养。【结果】暴露于空气中冻存菌株 0 7h后失去生长能力 ,0 8h开始出现球形菌 ,6 6 5h全部球形样变 ;平板上菌株于 1 9h开始出现球形菌 ,8 2h后失去生长能力 ,33 2h全部球形样变 ,84 3h后完全失去尿素酶活性 ;采用改良布氏肉汤 ,冻存菌株冻融后 2 2 4h及平板上菌株暴露于空气后 5 6 4h仍能复苏生长 ;球形样变细菌经改良布氏肉汤培养后 ,部分细菌重新恢复弧状或S形。【结论】改良布氏肉汤培养基能使发生球形样变的幽门螺杆菌重新生长。更多还原

【Abstract】 Objective Observing morphological changes and growing characters of Helicobacter pylori(Hp); Studying the role of an improved broth medium in Hp growth. Methods Frozen and plate cultured Hp strains were exposed to air, and then were observed for morphological changes under light microscope and growing characters at different exposure time. Culturing the Hp strains which had already been exposed to air for a different time with the improved broth medium and Columbia plate respectively. Result When exposed to air, frozen strains were observed losing their growing capacity on Columbia plate in 0.7 hour, beginning to change into coccal shape in 0.8 hour, and completely transforming into coccal form in 66.5 hours. As to the Hp strains cultured on Columbia plate, they were observed beginning to change into coccal shape in 1.9 hour, losing growing capacity on Columbia plate in 8.2 hours, completely transforming into coccal form in 33.2 hours, and wholely losing urease activity in 84.3 hours. Cultured with the improved broth medium, the frozen and plate cultured strains could be revived after exposed to air for 22.4 and 56.4 hours respectively. A portion of coccal form bacteria could be reverted to spiral or “S” shape after cultured with the improved broth medium. Conclusion The improved broth medium could revive Helicobacter pylori from its transformed coccal form.更多还原