【摘要】 【目的】观察不同浓度的葡萄糖对大鼠腹膜间皮细胞p2 1WAF1表达的影响。【方法】大鼠腹膜间皮细胞在含糖13 8g/L和 38 6g/L的无血清培养基培养 2 4h后 ,用流式细胞仪分析细胞周期分布 ,用RT PCR法测定p2 1WAF1的表达水平。【结果】经含糖 13 8g/L和 38 6g/L培养基培养 2 4h后腹膜间皮细胞大多出现细胞肥大和停滞于细胞周期的G1期 ,以 38 6g/L组明显。高浓度葡萄糖刺激p2 1WAF1mRNA表达 ,38 6g/L组表达明显增高 (P <0 0 5 ) ;无血清常规培养基组和甘露醇组几乎无表达。【结论】高糖可刺激间皮细胞p2 1WAF1的表达 ,且可能与高糖作用下细胞肥大及G1期阻滞有关。更多还原

【Abstract】 Objective To investigate the effect of different concentrated glucose on the p21WAF1 expression in rat peritoneal mesothelial cells. Methods Rat peritoneal mesothelial cells were cultured with serum free DMEM medium containing different glucose concentrations (13 8 g/L and 38 6 g/L) for 24 hours. The cell cycle distribution was then investigated by flow cytometry.. The expression of p21WAF1 was detected by RT PCR. Results In the presence of 13 8 g/L and 38 6 g/L glucose concentrations, most of the cells became hypertrophic, and were arrested in G 1 phase of cell cycle, which were obvious in the 38 6 g/L. High glucose stimulated expression of p21WAF1 mRNA, which was significantly higher in the 3 86%(P<0 05). p21WAF1 mRNA expressions were absent in serum free normal glucose concentration groups and the D mannitol groups. Conclusion High glucose stimulated expression of p21WAF1, which might be related to the hypertrophy and arrest in the G 1 phase of mesothelial cells by high glucose.更多还原