【摘要】 【目的】探讨激活补体是否会引起心肌缺血损伤 ,以及膜结合型补体调节蛋白CD59对补体引起的心肌缺血损伤有无保护作用。【方法】 18只豚鼠离体工作心脏随机分为 :A组 (对照组 ) ,在灌注液中加入体积分数为 3%灭活人血浆 +酵母多糖 ;B组 ,在灌注液中加入 3%人血浆 +酵母多糖 ;C组 :在灌注液中加入CD59(45 0 μg) +3%人血浆 +酵母多糖。记录各组处理前及处理后 15、30、45和 6 0min的心外膜心电图ST段变化、冠脉流量 (CF)、心输出量 (CO)、左心室最大内压 (LVPmax)、左心室内压最大上升速率 (dp/dtmax)和心率 (HR)。【结果】A组处理前后各项观察指标无明显变化 ;B组在接受处理后心外膜心电图出现ST段抬高、HR增加 ,CF、CO、LVPmax和dp/dtmax均下降 ,这些变化以处理后 45min最明显 ;C组除处理后 45～ 6 0min时心率稍增快外 ,其余指标处理前后无明显变化。【结论】　激活补体可直接引起豚鼠工作心脏心肌缺血损伤 ,CD59对补体介导的心肌缺血损伤有保护作用。更多还原

【Abstract】 Objective To observe that activated human complements directly injure isolated quinea pig working hearts and CD 59 prevents it. Methods Isolated guinea pig working hearts perfused with 3% denatured human plasma and zymosan in group A (control), 3% normal human plasma and zymosan in group B and 3% normal human plasma and CD 59 (450 μg) and zymosan in group C, respectively. Of these 3 groups, epicardial electrocardiogram (ECG) and coronary flow(CF), cardiac output(CO), maximum left ventricular developed pressure (LVP max ),maximum left ventricular pressure rise rate (dp/dt max ) and heart rate(HR) were recorded before and after 15, 30 , 45 and 60min perfusion. Results No apparent change in all above parameters was seen ingroup A before and after perfusion ;elevation of ST segment,faster HR and decrease in CF, CO, LVP max and dp/dt max were observed in group B, most prominent at 45 min after perfusion; only slight increase in HR in all observed parameters was observed in group C at 45～60 min after perfusion. Conclusion Activated human complements directly injured isolated guinea pig working hearts and CD 59 prevents it.更多还原