【摘要】 观察原位PCR方法在弓形虫感染病原学检测中的效果。以RH株弓形虫速殖子感染小鼠后第 2、 4、 6天处死 ,取肝脏制备石蜡标本及冰冻切片标本 ,以直接法原位PCR扩增并检测石蜡标本中的弓形虫DNA ,与免疫组化方法检测冷冻切片标本的结果相比较。 18例直接原位PCR法检测标本中均得到阳性信号 ,病原体检出率为 10 0 % ,优于免疫组化法 (11/18)。直接法原位PCR为检测病理标本中的弓形虫DNA提供了一种新的可行方法。更多还原

【Abstract】 Aim To accomplish the etiological diagnosis of toxoplasmosis by in situ PCR(ISPCR) Methods Mice inoculated with 2×10 2 tachyzoites of Toxoplasma gondii(RH strain)were sacrificed 2、4、6 days after infection The livers were taken out and prepared as paraffin embedded sections and frozen sections Direct ISPCR was employed to amplify and detect the DNA of T gondii in paraffin embedded sections The results were compared with that of immunohistochemical detection in frozen sections Results DNA of T gondii were detected Form All the 18 secxions by using ISPCR,while(11/18)were positive by immunohistochemical detection Conclusions ISPCR could be applicable in detecting the DNA of T gondii in paraffin embedded sections as a new method更多还原