【摘要】 目的　探讨肝细胞癌DNA异倍体、nm2 3 H1、C erbB 2和p5 3癌基因蛋白表达与肝细胞癌侵袭性的关系及其临床意义。方法　采用DNA图像分析系统定量测定 5 2例肝癌患者的肝癌细胞的DNA含量 ,用免疫组化方法检测癌基因蛋白在肝癌中的表达。结果　DNA异倍体在≤ 5cm肝癌组为 5 0 .0 % (12 /2 4) ,在 >5cm肝癌组为 82 .1%(2 3/2 8) ;DNA异倍体与肝癌肝内转移及合并癌栓有关。肝癌伴有肝内转移者的nm2 3 H 1阳性率明显高于不伴肝内转移者。肝癌合并癌栓者的 p5 3蛋白阳性率高于不合并癌栓者。异倍体肝癌的nm 2 3 H1和 p5 3蛋白阳性率高于二倍体肝癌。C erbB 2阳性率在各组中未见明显差别。术后生存率可能与DNA异倍体、nm 2 3 H1和 p5 3蛋白的阳性表达有关。结论　DNA异倍体、nm2 3 H1和 p5 3蛋白阳性表达与肝癌侵袭性有较密切关系 ,检测这些指标有助于根据肝癌的个体差异选择治疗措施 ,提高疗效更多还原

【Abstract】 Objective To study the clinical significance of DNA content and expression of nm23 H1, C erb B 2 and p53 oncoproteins in hepatocellular carcinoma. [WT5”HZ]Methods [WT5”BZ]DNA content was measured after DNA feulgen’s dyeing, the expression of nm23 H1, C erb B 2 and p53 oncoproteins was detected immunohistochemically. [WT5”HZ]Results [WT5”BZ]The incidence of aneuploid DNA content was 50.0% (12/24) in ≤5 cm group, and was 82.1% (23/28) in >5cm group; aneuploid DNA content was related with liver metastasis and cancerous thrombosis. The expression of nm23 H1 in HCC with liver metastasis was higher than that without metastasis. The positive rate of p53 in HCC with canerous thrombosis was higher than that without cancerous thrombosis. The positive rates of nm23 H1 and p53 in HCC with aneuploid DNA content were higher than those with diploid DNA content. The positive rate of C erb B 2 did not have significantly difference between groups. Postoperative survival rates were possibly related with DNA content as well as expression of nm23 H1, and p53 oncoproteins. [WT5”HZ]Conclusion [WT5”BZ]Aneuploid DNA conten, expression of nm23 H1 and p53 oncoproteins are closely related with the invasiveness of HCC, and have remarkably clinical significance.更多还原