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人参高产发根无性系的筛选及其高效液体培养

Selection and High efficient Culture of High yielding Hair Root Clone of Ginseng

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【作者】 赵寿经杨振堂李昌禹臧埔赵家平

【Author】 Zhao Shoujing,Yang Zhentang,Li Changyu,Zang Pu,Zhao Jiaping (Institute of Special Animal & Plant Science,Chinese Academy of Agricultural Sciences,Jilin 132109)

【机构】 中国农业科学院特产研究所!吉林132109

【Abstract】 In the co culture of root explants of ginseng and A 4 strain of Agrobacterium rhizogenes,the hairy root of ginseng transformed by A 4 strain were induced.By means of determination for growth rate,the high yielding hair root clone R98111 was selected.It achieved the most growth rate on a liquid 1/2 MS medium (25℃,in a shaker at 110r/min )and its growth rate increased by 31.2 fold in 28 days.Agropine and mannopine were detected in the R98111 clone by high voltage paper electrophoresis.In the liquid fermented culture of 2.0 litre medium,the yield of the hairy roots R98111 clone amounted to 38.29g(dry weight)in 28 days.

【关键词】 人参Ri质粒转化高效培养
【Key words】 GinsengRi plasmidTransformationHigh efficient culture
【基金】 中国农业科学院国际合作基金资助项目
  • 【文献出处】 中国农业科学 ,SCIENTIA AGRICULTURA SINICA , 编辑部邮箱 ,2000年05期
  • 【分类号】S567.51
  • 【被引频次】20
  • 【下载频次】218
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