【摘要】 目的 :研究白桦三萜类物质 (TBP )体内抗肿瘤作用及其对荷瘤小鼠免疫功能的影响。方法 :利用小鼠体内移植瘤模型 ,测定TBP对小鼠黑色素瘤B16、肉瘤S180、Lewis肺癌和艾氏腹水癌等肿瘤的抑瘤率。采用放射性掺入法检测ConA诱导的脾细胞增殖反应 ;放射性释放法检测巨噬细胞细胞毒活性 ;中性红释放法检测TNF和NK细胞活性。结果 :TBP对各瘤株的抑瘤率均达到 30 %以上 ,对黑色素瘤B16的效果最好 ,抑瘤率为 5 1 4%。TBP对ConA诱导的增殖反应和NK细胞活性无明显影响 ,但可促进巨噬细胞和脾细胞分泌TNF ,增加巨噬细胞的细胞毒活性。结论 :TBP具有良好的抗肿瘤作用 ,增强机体的非特异性免疫功能是其抗肿瘤机制之一更多还原

【Abstract】 Objective:To study the antitumor activity and immunoregulatory effect of Triterpenes isolated from Betula Platyphylla (TBP) Methods: Inhibition rate (IR) on tumor growth was determined by the mice with transplantable tumors (melanoma B16, Sarcoma 180, Lewis lung carcinoma and Ehrlich ascites cancer ) , splenocyte proliferation induced by ConA was measured by 3H TdR incorporation ; cytotoxicity of macrophage was observed by 3 H TdR release; activity of TNF and NK cell was determined by dye (natural red)release Results: IR of 0 80 g/kg and 1 20 g/kg TBP on all of above tumor strains were greater than 30% in vivo TBP does not show significant effect of splenocyte proliferation and NK cell activity, but significantly promoted the activity of TNF producted by macroghage and splenocyte TBP also increase the cytotoxicity of macrophage Conclusion:TBP shows potent antitumor effect in vivo Promoting nonspecific immunoactivity is one of the antitumor mechanism of TBP更多还原