【摘要】 目的 :优化热聚IgG的制备条件 ,提高对RF的检测灵敏度。方法 :利用高效液相凝胶色谱和酶联免疫吸附技术研究加热条件对热聚IgG分子量分布及其对类风湿因子结合能力的影响。结果 :将IgG溶液 (10mg/ml)在 6 2℃热聚 30min ,其优势聚合度为 11左右 ,此时它对类风湿因子的结合能力最强。结论 :应用高效液相凝胶色谱与ELISA技术 ,对热聚IgG体系进行分离测定和活性分析 ,缩短了测定时间 ,提高了结果的灵敏度和准确性。更多还原

【Abstract】 Objective:To optimize the preparation conditions of heat aggregated IgG for increasing the determination sensitivity of RFs. Methods:Effect of heating conditions on molecular weight distribution of heat aggregated IgG(HAIgG)and its reactivity to rheumatiod factors(RFs) was studied by gel HPLC and ELISA,respectively.Results:The reactivity of HAIgG to RFs was correlative to size of aggregates and the optimal condition was to heat IgG solution (10 mg/ml) at 62℃ for 30 min.Conclusion:The authors made the MW determination and activity analysis for heat aggregated IgG system by applying gel HPLC and ELISA,the period of detection was decreased and the sensitivity and accuracy were improved.更多还原