【摘要】 目的　研究角膜中是否有单纯疱疹病毒 1型 (herpessimplexvirustype 1,HSV 1)功能性基因的潜伏。方法　在新西兰白兔角膜上制作典型的单纯疱疹病毒 1型引发的角膜炎 (herpessimplexkeratitis,HSK)模型 ,然后将进入稳定期的病变角膜移植到健康兔眼上 ,术后 2周取下植片。每个植片标本均分为三部分 ,一部分角膜片先行HSV 1抗原检测 ,另一部分采用多聚酶链反应 (polymerasechainreaction,PCR)技术检测HSV 1潜伏相关转录 (lactencyassociatedtranscript,LAT)、胸苷激酶 (thymidinekinase ,TK)和DNA聚合酶基因 ,第三部分经器官培养 3周后 ,再与兔原代肾 (rabbitkidney ,RK)细胞一起培养 1周 ,再检测培养后的RK细胞和角膜片中有无HSV 1抗原表达。结果　在 15个HSV 1抗原阴性的角膜片中 ,8个检测到LAT基因 ,10个有TK基因 ,6个有DNA聚合酶基因。 3份RK细胞和 6个培养后的角膜片HSV 1检测阳性。结论　HSV 1感染潜伏期的角膜内存在HSV 1功能基因。更多还原

【Abstract】 Objective To investigate the possible existence of herpes simplex virus 1 (HSV 1) functional gene latency in cornea Methods A herpes simplex keratitis (HSK) model was established in New Zealand White (NZW) rabbit corneas, latently infected corneas were transplanted to one eye of each naive NZW rabbit One week after the operation, the corneal buttons were removed and divided into three portions One portion was processed for HSV 1 antigen detection, another was for HVS 1 latency associated transcript (LAT), thymidine kinase (TK) and DNA polymerase gene detections by polymerase chain reaction (PCR), and the last portion after tissue culture for 3 weeks, it was co cultured with rabbit kidney cells (RK) for one week, then the expression of HSV 1 antigen in the RK cells and this portion of corneal button was detected Result In the 15 corneal samples negative for HSV 1 antigen, LAT gene was detected in 8 samples, TK gene in 10 and DNA polymerase gene in 6 samples HSV 1 antigen was detected in 3 samples of RK cells and 6 cultured corneal samples Conclusion HSV 1 functional genes are retained in latently infected cornea更多还原