【摘要】 目的　了解深圳地区无偿献血人群人类免疫缺陷病毒 (HIV)感染流行情况。方法　采用酶联免疫吸附试验 (ELISA)对无偿献血者进行抗 -HIV - 1/2筛查 ,对初查阳性标本用聚合酶链反应 (PCR)方法进一步检测外周血单个核细胞 (PMBCs)中HIVDNA和血浆中HIVRNA ,并对 2例无偿献血者PCR扩增阳性片段进行序列测定。结果　在 46 0 95例无偿献血者中 ,发现抗 -HIV1阳性 7例 ,阳性率为 0 .0 15 % ;在 7例抗 -HIV - 1阳性献血者的PBMCs中均检出HIVDNA阳性 ,5例血浆中检测出HIVRNA阳性 ;2例献血者HIVDNA测序分析显示 ,感染的是HIV - 1E亚型毒株。结论　深圳地区无偿献血者人群存在HIVE亚型毒株感染 ,对无偿献血者仍应加强HIV感染的筛查和监测更多还原

【Abstract】 Objective To study the prevalent status of HIV-1 in population of non-remunerated blood donors in Shenzhen.Methods 46 095 non-remunerated blood donors were tested for anti-HIV-1/2 by ELISA. The donors of anti-HIV positive were further detected for HIV DNA from peripheral blood mononuclear cells (PMBCs) by nested-PCR and HIV RNA from plasma by RT-PCR. The partial genome of env of 2 blood donors were sequenced.Results The anti-HIV-1 was tested positive in 7 of 46 095 voluntary blood donors and the positive rate was 0.015%. In these 7 non-remunerated blood donors of anti-HIV-1 positive,7 were positive for HIV DNA in PMBCs and 5 positive for HIV RNA in plasma. The sequence analysis showed that 2 donors were infected by HIV-1 subtype E strains.Conclusion There exists HIV-1 subtype E infection in population of non-remunerated blood donors in Shenzhen.It is essential to detect and monitor strictly the population of non-remunerated blood donors.更多还原