【摘要】 目的　研究罗哌卡因对豚鼠心室肌细胞膜离子通道的影响 ,探讨其心脏毒性作用的发生机理。方法　用酶解法获得单个的左心室肌细胞 ,用标准的全细胞膜片钳技术记录用药前后的钠流 (INa)、L 型钙流 (ICa L)、延迟整流性钾流 (IK)和内向整流性钾流 (IK1)的变化。结果　罗哌卡因对INa及ICa L都有抑制作用 ,且呈浓度依赖性。 5 0 μmol L 1罗哌卡因可使INa、ICa L分别下降 36 8%、7 6 8% ;10 0 μmol L 1罗哌卡因可使INa、ICa L分别下降 6 6 1%、2 3 13% ;对INa的抑制作用强于ICa L(P <0 0 5 ) ,10 0 μmol L 1罗哌卡因对IK及IK1无影响 (P >0 0 5 )。结论　罗哌卡因的心脏毒性作用与抑制钠通道和钙通道有关。更多还原

【Abstract】 Objective To study the effect of ropivacaine on ion channels in isolated ventricular myocytes Methods Single ventricular cell of guinea pig was obtained by an enzymatic dissociation method The whole cell configuration of the patch clamp recording technique was used to record the changes of sodium current(INa),L type calcium current(ICa L), delayed rectifying K + current(IK) and inward rectifying K + current(IK 1) following the administration of ropivacaine at 50μmol L 1 and 100μmol L 1 Results Ropivacaine reduced the amplitude of INa and ICa L in a dose dependent manner INa and ICa L were decreased by 36 8% and 7 86% respectively after administration of 50μmol L 1 ripovacaine Ropivacaine 100μmol L 1 decreased the amplitude of INa and ICa L by 66 1% and 23 13% Ropivacaine 100μmol L 1 did not change the amplitudeof IK and IK 1 (P>0 05) Conclusion The cardiotoxicity of ropivacaine is related to the inhibition of sodium and calcium channels更多还原