【摘要】 目的　揭示北京地区啮齿类动物所携带的流行性出血热 (EHF)汉坦病毒的基因型、毒株差异 ,追溯其传染来源。方法　本研究应用逆转录 聚合酶链反应 (RT PCR)扩增技术及核苷酸序列测定方法对免疫荧光抗原阳性的标本进行研究分析。结果　对 5个阳性标本的 30 0bp部分M基因组片段序列分析表明均为SEO型汉坦病毒 ;毒株核苷酸序列比较表明 :与其他SEO型毒株同源性均达 94%以上 ,而与其他HNT型毒株同源性均低于 72 % ;毒株序列所构建的系统发生树显示 ,北京地区SEO型汉坦病毒可能至少存在 4个不同的进化分支和两个不同的汉坦病毒亚型。结论　北京地区流行性出血热流行的主要毒株是SEO型汉坦病毒 ,毒株间存在着一定的基因差异 ,其传染来源有待深入研究。更多还原

【Abstract】 Objective To understand the genetic type of Hantaviruses and the difference between them caused by rodents in Beijing and to furhter explore the source of the infectious factors. Methods Hantavirus RNA, isolated from lungs of rodents captured in Beijing and positive with Hantavirus antigens with frozen sectioning and Immunofluorescent assay, were reverse-transcribed and amplified with PCR with Hantavirus-specific primers. Results Five of the PCR amplifications were discovered and sequenced with 300 bp sequence data of M segments (from 2003-2302nt according cDNA of seoul 8039 strain). Nucleotide sequence homology showed that they were sequences of SEO-type Hantavirus. Compared with SEO type Hantavirus, the nucleotide sequence homology of these samples was more than 94% while the homology of amonia acid sequence was more than 98%. When compared with HNT type Hantavirus, the homology of nucleotide sequence became less than 72% with the homology of amonia acid sequence less than 81%. Similar to other Hantavirus of SEO type, their nucleotide sequences and deduced amino acid sequences were highly preserved. Phylogenetic tree analysis showed that the five viruses could be divided into at least 4 branches. Conclusion It was quite likely that there were at least two sub-type SEO viruses with 4 branches that were circulating in Beijing.更多还原