【摘要】 目的　观察载脂蛋白AI基因多态性频率在动脉粥样硬化性脑梗死患者中的分布,探讨基因型与脑梗死的关系。方法　用聚合酶链反应技术(PCR)检测北京地区199例脑梗死患者及204例健康人的载脂蛋白AI基因启动子(MspI1,-75bp)和第一内含子(MspI2,+83bp)两个MspI酶切位点限制性片段长度多态性(RLFP)。结果　北京地区403例受检者中载脂蛋白AI基因启动子均以M1++和M1+-为主要基因型,第一内含子大多数为M2++基因型;脑梗组M1+-基因型频率分布明显高于对照组(0.487vs0.368,P<0.05,OR=1.64);脑梗组M1+-/M2++单体型基因型频率显著高于对照组(0.437vs0.314,P<0.05,OR=1.70)。结论　ApoAI基因5′端MspI位点M1+-基因型、M1+-/M2++单体型基因型可能与动脉粥样硬化性脑梗死发病有一定的关系更多还原

【Abstract】 Objective To investigate the frequency of the restrict fragment length polimorphisms (RFLP) at 2 MspI sites in the 5′-end of apolipoprotein AI (ApoAI) gene and its potential interaction to atherosclerotic cerebral infarction (ACI) in Chinese Han population. Method Polymerase chain reaction (PCR) technique was used in a sample of 199 cases with documented ACI and 204 healthy matched by age and sex individuals selected from Chinese Han nationality in Beijing. The studied loci include promoter region (-75 bp) and the intron-1 (+83 bp). Results ⑴Both ACI and control groups M1++ and M1+- were major frequent genotypes in Beijng area,M2++ were major frequent genotypes in intron-1; ⑵ M1+- genotype appeared more common among patients than in controls ( 0.487 vs 0.368 , P < 0.05 , OR = 1.64 ); ⑶ Individuals with M1+-/M2++ haplotype were significantly increased in patients campared with controls ( 0.437 vs 0.314 , P < 0.05 , OR = 1.70 ). Conclusion It is suggested that both M1+- genotype and M1+-/M2++ haplotype of ApoAI gene might associate with ACI in our study.更多还原