【摘要】 目的　探讨反义寡脱氧核苷酸 (ASODN)在不同作用时点对人胰腺癌细胞系PaTu8988s抑制作用的影响。方法　用人工合成的与K ras互补的ASODN在不同作用时点作用于指数生长期人胰腺癌细胞系PaTu8988s,并用四甲基偶氮唑盐 (MTT)法作活细胞测定。结果　人胰腺癌细胞系PaTu8988s经 5 0 μg/ml和 10 0 μg/mlASODN处理后 ,在 12h、2 4h、48h和 72h的抑制率分别为42 .2 5 %、6 6 .2 9%、6 9.5 5 %、74.5 8%和 6 6 .2 0 %、91.43 %、98.18%、98.33 %。结论　抑制效应从 12h开始出现 ,48～ 72h最为明显 ,其后出现时间和效率的饱和现象 ;抑制率的高峰期随着浓度增大而前移更多还原

【Abstract】 Objective To study the effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s. Methods Human pancreatic cancer cell line PaTu8988s in exponent growth stage are adopted. We observe the effect of different time point on the PaTu8988s cell at 12、24、48 and 72 hour. Results The inhibitory rate on PaTu8988s cell line is 42.25%、66.29%、69.55%、74.58% and 66.20%、91.43%、98.18%、98.33% for ASODN concentrations of 50 μg/ml and 100 μg/ml at 12、24、48 and 72 hour, respectively. Conclusion The inhibitory effect of ASODN began from 12 hour and becomes more obvious at 48～72 hour. The higher the concentration of ASODN, the earlier the peak of inhibited rate.更多还原