【摘要】 目的　探讨ras raf信号转导途径在受辐射KG 1细胞中对周期的调控作用方式。方法　通过转染DN ras阻断GM CSF的信号传递后 ,瞬间转染cyclinD1,观察cyclinD1对受辐射细胞周期阻滞的影响作用。结果　转染DN ras的受辐射KG 1细胞即使用GM CSF刺激亦不能跳出G1期阻滞 ;瞬间转染cyclinD1能促进受辐射细胞跳出周期阻滞。 结论　ras raf信号转导途径通过促进周期蛋白cyclinD1的表达而对受辐射细胞周期进行调控。更多还原

【Abstract】 Objective\ To investigate the way of ras\|raf signaling pathway which regulate the G 1 phase in irradiated KG\|1 cells. Methods\ Blocked the GM\|CSF signaling pathway by transfected DN\|ras and then momentary transfected cyclin D1 into irradiated KG\|1 cells,the effects of cyclin D1 on G 1 phase was examined. Results\ The irradiated KG\|1 cells transfected DN\|ras can’t recover form G 1 phase arrest even though the GM\|CSF was given,momentary transfected cyclin D1 promote the irradiated KG\|1 cells from G 1 arrest. Conclusion\ Activation of ras\|raf signaling pathway regulate the cell cycle of the irradiated KG\|1 cells through promotion the expression of the cyclin D1.更多还原