【摘要】 目的　了解放射治疗协同单纯疱疹病毒 胸苷激酶 (HSV TK) /更昔洛韦 ( gancyclovir,GCV)对膀胱癌细胞的联合杀伤作用。方法　通过逆转录病毒介导 ,将潮霉素磷酸转移酶和HSV TK的融合基因 (HyTK)基因转入膀胱肿瘤细胞株EJ中并获得表达 ,联合应用放射治疗及低剂量GCV ,了解基因 放射治疗对转基因细胞EJ/HyTK的杀伤作用。 结果　SouthernBlot、DotBlot检测证实HyTK在转基因细胞的表达 ,联合应用低剂量GCV可使转入HyTK基因的膀胱癌细胞株对放疗的敏感性明显增高 (SER =1.3 6)。结论　放疗协同HSV TK/GCV系统可作为膀胱癌基因治疗的一种有效方法。更多还原

【Abstract】 Objective To explore the killing effect of retrovirus mediated HSV TK/GCV system combined with radiotherapy on human bladder carcinoma cells. Methods By using retrovirus mediated gene transfer technique,hygromycin phosphotransferase thymidine kinase fusion gene (HyTK) was transferred into EJ bladder carcinoma cells. Antitumor effects were observed after irradiation with 60 Co γ rays as well as gancyclovir treatment. Results EJ bladder carcinoma cells transduced with HyTK gene and treated with low concentrations of gancyclovir,which alone showed little cytotoxicity,were highly sensitive to radiation. The sensitizer enhancement ratio (SER) was 1.36. Conclusion The combination of retrovirus mediated HyTK/GCV gene therapy with standard radiation therapy might improve the therapeutic effectiveness for bladder carcinoma in clinical practice.更多还原