【摘要】 目的　探讨肿瘤坏死因子α(TNF α)基因多态性与多囊卵巢综合征 (PCOS)发病的关系。方法　用聚合酶链反应、限制性内切酶消化及电泳技术 ,对 118例PCOS患者和 5 4例对照者TNF α基因启动子区 30 8位碱基多态性进行检测。出现 87/87片段定义为TNF1基因型 ,出现 87/10 7和 10 7/10 7片段定义为TNF2基因型。比较两种基因型在PCOS患者和对照者的分布及其与PCOS患者糖代谢特征的关系。结果　TNF1和TNF2基因型在PCOS患者分别为 74.6 %、2 5 .4% ,和对照者 (6 8.5 %和31.5 % )的分布比较 ,差异无显著性 (P >0 .0 5 )。PCOS患者中 ,肥胖者 44例 ,非肥胖者 74例 ;肥胖TNF1基因型患者胰岛素敏感性 (指数为 0 .0 14± 0 .0 0 2 )显著低于肥胖TNF2基因型患者 (指数为 0 .0 5 1± 0 .0 34) (P <0 .0 5 ) ,其他糖代谢指标在两者无显著差异 ;而非肥胖TNF1基因型患者和非肥胖TNF2基因型患者之间各指标都没有显著差异。肥胖TNF1基因型患者糖耐量各指标均与非肥胖TNF1基因型患者有显著差异 (P <0 .0 5 ) ;而肥胖TNF2基因型患者与非肥胖TNF2基因型患者的空腹血糖和胰岛素、6 0min血糖、葡萄糖曲线下面积以及胰岛素敏感指数在两者间差异无显著性 (P >0 .0 5 )。结论　TNF α基因 30 8位碱基多态性与PCOS无明显相关性 ,但TNF2更多还原

【Abstract】 Objective To investigate the relationship between 308 bp polymorphism in tumor necrosis factor α (TNF α) gene promoter region and the pathogenesis of polycystic ovary syndrome (PCOS). Methods One hundred eighteen Chinese women with PCOS (44 were obese,and 74 nonobese) and 54 controls were genotyped according to the fragment length [87bp and(or) 107 bp] of TNF α gene promoter by the technique of polymerase chain reaction restriction fragment length polymorphism. The distribution of TNF1 genotype (87/87) and TNF2 genotype (87/107+107/107) in the two groups and their relations to parameters of glucose metabolism associated with PCOS were compared. Results There was no significant difference in the distribution of TNF1 and TNF2 genotypes between PCOS group and the controls ( P >0.05). In obese PCOS group, only significant lower insulin sensitivity index was found in TNF 1 genotype as compared with TNF2 genotype ( P <0.05).In nonobese PCOS group, there was no significant difference in all parameters of glucose metabolism between TNF1 and TNF2 genotype. All parameters of glucose metabolism in obese patients with TNF1 genotype were significantly different from those in nonobese with TNF1 genotype. However, significant differences were found only in some parameters when comparison was made between obese and nonobese within the TNF2 genotype group. Conclusion There is no association between TNF α gene 308 bp polymorphism and the pathogenesis of polycystic ovary syndrome, but PCOS patients with TNF2 genotype may associate with improvement to certain degree on abnormal glucose tolerance usually existed in obese PCOS women.更多还原