【摘要】 目的　研究丙型肝炎病毒 (HCV)准种特性与感染慢性化的关系 ,以及个体准种特性的来源形式。方法　收集HCVRNA阳性的 10例急性丙肝、2 0例慢性丙型肝炎 (丙肝 )和 11例肝细胞癌 (HCC)患者 ,采用单链构型多态性分析 (SSCP)方法进行HCV准种检测。结果　急性丙肝、慢性丙肝和HCC患者中 ,SSCP电泳条带数分别为 2 .7± 1.16、4.8± 1.6 8和 5 .2± 2 .85。慢性丙肝和HCC的条带数显著高于急性丙肝 (P <0 .0 5 )。进行DNA序列分析研究发现 ,准种高变区间的变异性显著低于本地株间和异地株间的变异性 (P <0 .0 1)。结论　SSCP是检测准种相对简便而有效的方法。HCV准种特性与其感染慢性化相关。HCV感染个体准种的来源主要为感染过程中的核酸突变。更多还原

【Abstract】 Objective To study the source of hepatitis C viral quasispecies and its relationship with chronic infection of hepatitis C virus (HCV).Methods Viral quasispecies from 10 cases of acute hepatitis C,20 cases of chronic hepatitis C and 11 cases of hepatic cellular cancer (HCC) patients with serum HCV RNA positive were detected by single strand conformation polymorphism (SSCP) analysis.Results 2.7±1.16,4.8±1.68 and 5.2±2.85 SSCP bands were detected in the patients with acute hepatitis C, chronic hepatitis C and HCC respectively. The number of viral quasispecies in chronic hepatitis C and HCC with HCV infection were significantly higher than that in acute hepatitis C ( P <0.05). The variance of high variable region in quasispecies was found to be much smaller than that in local isolates and distant isolates by DNA sequencing analysis (6.50±2.00 vs 20.33±2.31 and 24.33±4.04 respectively, P < 0.01). Conclusion SSCP analysis is an efficient and relatively simple method for detection of viral quasispecies. Hepatitis C viral quasispecies are strongly related with chronic infection. The source of HCV quasispecies from infected individuals may originate from viral genome mutation during persistent infection.更多还原