【摘要】 目的　探讨反义人端粒酶RNA(hTR)对胃癌细胞的生长抑制作用。方法　将反义hTR真核表达载体经脂质体介导转染人胃癌细胞系SGC790 1,体外培养及接种裸鼠观察其基因转染细胞的细胞周期、超微结构变化、生长速率及致瘤性。结果　反义hTR在潮霉素筛选的阳性克隆中稳定表达 ,正义hTR表达下调 ,并出现凋亡改变。基因转染细胞体外传代培养的生长速率大多减慢 ,在裸鼠皮下的致瘤性明显降低。荷瘤鼠存活时间延长。结论　反义hTR可以通过诱导细胞凋亡逆转人胃癌细胞的恶性生长更多还原

【Abstract】 Objective To observe the inhibitory effects of antisense human telomerase RNA(hTR) on growth of human gastric cancer cells. Methods SGC7901 cell line was transfected with antisense hTR expression vector (pBBS hTR) by lipofectAMINE. The gene transfected cells were cultured in vitro and then inoculated into nude mice. Results Antisense hTR expression was enhanced and sense hTR expression inhibited in clones containing antisense hTR after hygromycin resistant selection which induced tumor cells apoptosis. The growth rate of the major gene transfected cells in culture and tumorigenesis of the transfected cells in nude mice were significantly reduced in comparison with those of controls. The survival time of nude mice inoculated with the gene transfected cells was markedly prolonged when compared to controls. Conclusions The malignant proliferation of gastric cancer cell can be reversed by antisense hTR induced cell apoptosis. Thus antisense hTR may serve as a potential therapeutic approach to the treatment of human cancer.更多还原