【摘要】 采用直线加速器照射制作动物的β损伤模型,同时对ΝΙΗ 3Τ3细胞进行β外照射,观察胶原总量及Ⅰ,Ⅲ型胶原含量的改变;观察胶原降解酶ΜΜPs-1活性变化;同时测定损伤后细胞因子ΤGF-β1,IL-6的变化情况。结果表明,β损伤后胶原总量变化不大,而Ⅰ型胶原含量下降,Ⅲ型胶原含量上升;MMPs-1 活性上升;β损伤后TGF-β1,IL-6 表达量增加。提示胶原代谢的变化在放射性皮肤损伤中起关键作用,损伤后胶原代谢的调控机制中,TGF-β1 和 IL-6 起重要作用。更多还原

【Abstract】 The animal model of β radiation injury was established by the β radiation produced by the linear accelerator; and irradiated NIH 3T3 cells were studied. In the experiment the contents of total collagen, collagen type Ⅰand type Ⅲwere measured. The activity of MMPs-1 were tested. The contents of TGF-β1, IL-6 were also detected. The results showed that after exposure to β radiation, little change was found in the content of total collagen, but the content of collagen Ⅰ decreased and the content of collagen Ⅲ, MMPs-1 activity increased; the expression of TGF-β1, IL-6 increased. The results suggest that changes in the metabolism of collagen play an important role in the irradiated injury of the skin; TGF-β1, IL-6 may be essential in the regulation of the collagen metabolism.更多还原