【摘要】 目的 :建立了测定薤白中呋甾皂苷含量的E试剂比色法。方法 :用对二甲氨基苯甲醛 HCl乙醇液的发色体系 ,测定波长 5 15nm ,利用均匀设计原理确定最佳实验条件。结果 :标准曲线在 0 .1975～ 0 .9880mg·ml-1线性关系良好 ,平均回收率为 97.7% (RSD =3.6 5 % )。结论 :此法可作为评价薤白的质量标准。更多还原

【Abstract】 Objective:To establish an Ehrlich reaction colorimetry method for determining furostanol saponins in Bulbus Allii Macroste.Method:Based on the coloration reaction between furostanol saponins and p dimethylaminobenzaldehyde,the UV detection wavelength used was 515 nm,and the optimum conditions were selected on homogeneous design principle. Result:The standard curve of macrostemonoside G waslinear in the concentration range of 0.1975～0.9880 mg·ml -1 .The average recovery was 97.7%( RSD =3.65%).Conclusion:The method is rapid,accurate and easy to operate. [更多还原