【摘要】 建立了血浆中盐酸奈福泮的HPLC测定法。血浆样品采取液 液两步提取后 ,以ODS HypersilC18为固定相 ,35 %乙腈∶6 5 % 0 .0 1mol/L庚烷磺酸钠 (pH =3 .0 )为流动相。紫外检测波长 2 15nm。最低检测浓度为 2 .5ng/ml,线性范围 2 .5～ 5 0 0 .0ng/ml(r =0 .9995 ) ,四种浓度的回收率大于 90 % ,日间日内变异系数小于15 %。更多还原

【Abstract】 An reversed phase HPLC method was established for determination of nefopam in plasma. The drug was extracted from plasma by liquid-liquid phase. ODS-Hypersil C 18 column was used. Mobile phase consisted of 35% acetonitrile∶65% 0.01 mol/L sodium heptane sulfonate(pH=3.0).The eluted peaks were detected by UV detector at 215 nm. The recoveries of nefopam from plasma were larger than 90% and RSD of intra-day and inter-day were smaller than 15% . Calibration curves were linear over 2.5～500.0 ng/ml(r=0.9995) and the minimum quantitative concentration was 2.5 ng/ml. The method was successfully used to study pharmcokinetics of nefopam in both dog and human.更多还原