【摘要】 ＥＩＡＶ在繁殖过程中的转录涉及到多种因子的调节，其中ＴＡＴ蛋白是病毒编码的反式激活因子，是病毒复制必须成分。ＴＡＴ是非结构蛋白，由病毒基因组全长转录产物经多次拼接而成。本研究使用ＥＩＡＶ疫苗毒感染驴巨噬细胞培养物，在病毒增殖早期提取细胞总ＲＮＡ，反转录后使用中国ＥＩＡＶ毒株特异引物扩增病毒基因组拼接产物。将扩增产物克隆后经过核苷酸序列分析，和与基因组全序列的比较。确定了编码ＥＩＡＶ反式激活蛋白的转录产物及阅读框架及转录后拼接位点，研究发现至少有两种拼接产物编码ＴＡＴ。更多还原

【Abstract】 The transcription of EIAV was regulated by several viral and cellular factors; TAT is a transactivating protein encoded by the virus genome and it is necessary for virus replication. mRNAs encoding TAT were spliced from full length viral transcript in the host cell. In this study, donkey macrophage culture was infected with donkey leukocyte attenuated EIAV. At the early stage of virus replication total RNA was extracted from infected cells. After reverse transcription, polymerase chain reaction was performed with EIAV specific primers.Then PCR products were cloned in the pUC19 plasmid vector and the inserts were sequenced. Two clones encoding TAT were obtained in this study. ORF encoding TAT and splicing sites were also determined.更多还原