【摘要】 用败血支原体 (MG)A5 96 9株制备ELISA抗原 ,与抗鸡IG单抗IB7酶结合物建立了检测鸡血清抗体水平的间接ELISA方法 ,交叉试验、阻断试验、重复性试验等表明该方法重复性好、特异性强 ,灵敏度高。确立了将鸡血清 6 4倍稀释监测ELISA效价 (ET)的回归方程y=1 .383+0 .2 2 4x(P >0 .0 5 ) ,可用于定量测定 ,血凝抑制试验 (HI)与ELISA方法的比较试验表明 ,ELISA法比HI试验敏感性高 4倍以上。更多还原

【Abstract】 The indirect ELISA method for detecting CRD antibody level were developed by preparing coating antigen using MG A5969 strains and enzyme conjugate of mouse against light chain of chicken Ig.The method have a strong repeatibility, a high specificity,and good sensitivity.The qualitatively detect titer of interrelated equation y=1.383+0.224x(p>0.05)were got by using 64_fold dilution sera sample.The ELISA titer is 4 times higher than Heamagglutination_inhibition test(HI).更多还原