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松杉灵芝菌丝体多糖（FI0-c）对人组织瘤细胞和人外周血白细胞产生炎症性细胞因子的影响（Ⅱ）（英文）

Effects of polysaccharides （FI0-c） from mycelium of Ganoderma tsugae on proinflammatory cytokine production by THP-1 cells and human PBMC （II）

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【作者】 高晓霞； 王本祥； 费晓方； 张洁； 宫毓静； 南陆彦； 永田年； 池岛乔；

【Author】 GAOXiao-Xia, WANG Ben-Xiang, FEI Xiao-Fang, ZHANG Jie, GONG Yu-Jing, MINAMI Mutsuhiko, NAGATA Toshi, IKEJIMA Takashi (Research Center of New Drug, Changchun College of Traditional Chinese Medicine, Changchun 130021; Department of Pharmaceutical Analysis, Guangdong College of Pharmacy, Guangzhou 510224; College of Life Sciences, Jilin University, Changchun 130012; Department of Chinese Pharmaceutical Analysis Changchun College of Traditional Chinese Medicine, Changchun 130021, China; Department of Parasitology, Yokohama City University School of Medicine, Yokohama 236 - 0004, Japan; Department of Microbiology , Hamamatsu Medical University, Hamamatsu 431 - 3192, Japan)

【机构】 长春中医学院新药研究中心； 长春中医学院中药系分析化学教研室； 横滨市立大学医学部寄生虫学教研室； 滨松医科大学微生物学教研室； 长春中医学院新药研究中心 长春 130021 广东药学院药学系药物分析教研室； 广州 510224； 长春 130021； 长春 130021 吉林大学生命科学院； 长春 130012； 长春 130021 中国； 横滨市 236-0004 日本； 滨松市 431-3192 日本； 长春 130021；

【摘要】 目的:比较研究水溶性多糖（FI0-c）及其氯磺酸修饰产物（FI0-c-S）对人炎症性细胞因子产生的影响.方法:应用氯磺酸修饰法对多糖进行化学修饰.用放射免疫分析法（RIA）及逆转录聚合酶链反应（RT-PCR）测FI0-c和FI0-c-S对人组织瘤细胞（THP-1）和人外周血单核细胞（PBMC）分泌各种与炎症有关的细胞因子,白介素-1（IL-1α）和肿瘤坏死因子α（TNFα）的影响及对mRNA表达的影响.结果:FI0-c和FI0-c-S（浓度分别为4,40,400 mg/L）显著提高 低剂量组LPS 10 mg/L协同PMA 200 nmol/L诱导的THP-1细胞产生TNFα的量,然而,这些多糖明显地抑制高剂量组LPS 100 mg/L协同PMA诱导的THP-1细胞产生TNFα.在无刺激的条件下FI0-c能够诱导比较多量的IL-1α产生,但是FI0-c或FI0-c-S却都明显抑制高剂量或低剂量LPS和PMA诱导的THP-1细胞产生IL-1α.低浓度FI0-c 4 mg/L显著抑制高剂量组LPS 100 mg/L协同PMA诱导的THP-1细胞产生IL-1或TNFα mRNA及蛋白质的量.结论:松杉灵芝菌丝体水溶性多糖在不同的刺激条件下具有双向免疫调节作用.化学修饰的多糖可改变原多糖对细胞因子产生的调节方向.

【Abstract】 AIM: To study the effects of water-soluble polysaccha-rides, FI0-c, and its sulfated derivative, FI0-c-S, on production of human proinflammatory cytokines, interleukin-1α (IL-1α) and tumor necrosis factor a ( TNFα). METHODS: The herbal polysaccharides were modified by chlorosulfornic acid in dimethyl sulfoxide (Me2SO). Cytokine production was measured by radioimmunoassay. mRNA for the cytokines was measured by semi-quantitative RT-PCR. RESULTS: FI0-c 4 mg/L itself induced IL-lα production by THP-1 cells without stimulants, such as lipopolysaccharides (LPS) and phorbol myristate acetate (PMA). On the other hand, FI0-C and FI0-c-S inhibited the IL-lα production by THP-1 cells with these stimulants. FI0-C and FI0-c-S significantly upregulated TNFα production by THP-1 cells without stimulants or at a low dose of LPS 10 mg/L and PMA 200 nmol/L, whereas these polysaccharides markedly downregulated the TNFa production by a high dose of LPS 100 mg/L and PMA. Human peripheral blood mononuclear cells (PBMC) responded to FI0-c and FI0-c-S in IL-lα and TNFα production in a fashion similar to THP-1 cell re-sponses. FI0-c 4 mg/L downregulated high-dose LPS-and PMA-induced IL-lα or TNFα mRNA and their protein production by THP-1 cells. CONCLUSION: The water-soluble polysaccharides of Ganoderma tsugae mycelium have bidirectional immunomodulatory effects on cytokine production in different cell stimulatory conditions. Chemical modification of this polysaccharide changed the intensity of regulatory effect on cytokine production .

【关键词】 松杉灵芝； 多糖类； 白介素-1； 肿瘤坏死因子；
【Key words】 Ganoderma tsugae； polysaccharides； interleukin-1； tumor necrosis factor；
【基金】 Project supported by the National Natural Science Foundation of China, No 39077847, ;Japan China Medical Association and Human Science Foundation.
  • 【文献出处】 Acta Pharmacologica Sinica ,中国药理学报(英文版) , 编辑部邮箱 ,2000年12期
  • 【分类号】R285
  • 【被引频次】3
  • 【下载频次】117
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