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阿卡斑(赤羽病)病毒的分离与初步鉴定

Isolation and Preliminary Identification of Akabane Virus

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【作者】 李其平周立桥姚龙涛蔡玉书陈向前卫龙兴李昌琳王新丁江李金萍李业红张雪莲

【Author】 Li Qi-Ping, Zhou Li-qiao, Yao Long-tao, Cai Yu-shu, Chen xiang-qian, Wei -xing, Li Chang-lin, Wang kin, Ding Jiang, Li Jin-Ping, Li Ye-hong, Zhang Xue-lian (Animal Quarantine institute Ministry of Agriculture, Qingdao 266032) shanghai Fengxian County Ani

【机构】 农业部动物检疫所!青岛266032上海市奉贤县畜牧兽医站农业部动物检疫所!?

【摘要】 1998年7月-8月从上海地区采集的蚊、蠓等标本中分离到12株病毒,其中有3株电镜观察病毒近似球形,有囊膜,病毒对乙醚、氯仿和胰蛋白酶敏感,对酸、热不敏感,抵抗5-氟脱氧尿苷。经血清-细胞和动物中和试验鉴定,该三株病毒均能被AKV病毒高效价免疫血清中和,其余9株病毒未被AKV高价免疫血清中和,经初步鉴定证实上述三株病毒为阿卡斑病毒(Akabane virus, AKV)。阿卡斑病毒在我国的分离尚属首次报道,具有重要的动物流行病学意义。

【Abstract】 isolates were Obtained from 30 000 mosquitoes collected in Fengxian Country, Shanghai during July and AUgUst 1998 and 3 of them were of spheriform with envelop Under EM. They were sensitive to ether, chloroform and sin, not sensitive to acid and heat treatment and resistant to floxuridine. The three isolates induced CPE in SVP,Vero and BHK-21 cells and were neutralized by AKV hyperimmune serum, while the other nine isolates were not. It was preliminarily demonstrated that the three isolates were Akabane virus. It is the first report on the isolation and identification of the virus in China, having important epizootiological significance.

【关键词】 阿卡斑病毒上海
【Key words】 Akabane virus Mosquito Shangh?
  • 【文献出处】 中国动物检疫 ,Chinese Journal of Animal Quaranitine , 编辑部邮箱 ,2000年07期
  • 【分类号】S855
  • 【被引频次】39
  • 【下载频次】222
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