【摘要】 目的　构建人类乳头瘤病毒 (HPV) 16型晚期基因 L1的原核表达质粒 ,以期从中选出能够高效表达 HPV16 L1的重组子 ,为进一步表达 L1蛋白奠定基础。方法　应用定向克隆策略 ,将 HPV16 L1基因分别克隆到原核表达载体 p Trac99A和 p ET- 2 1c中。结果　通过酶切鉴定选出了重组子 p Trc99A- HPV16 L1和 p ET- 2 1c-HPV16 L1。结论　 HPV16 L1的原核表达质粒构建成功更多还原

【Abstract】 Objective To construct prokaryotic expression plasmid of Human Papillomavirus type 16(HPV16)L1 gene from which we may select recombinant that can express HPV16 L1 in a high level,and which may make basis for gene expression of L1.Methods The L1 gene of HPV16 Chinese strain is cloned into prokaryotic expression vector pTrc99A and pET 21c by means of determined direction strategy.Results Recombinant plasmid,pTrc99A HPV16L1 and pET 21c HPV16L1,was screened by digestion of restrict endonuclease.Conclusions The construction of prokaryotic expression plasmid of HPV16L1 was achieved.更多还原