【摘要】 目的 :测定升压灵注射液在家兔体内药代动力学参数。方法 :采用高效液相色谱法 ,以升压灵注射液中辛弗林为指标进行测定。流动相为甲醇 水 (5 5∶45 ,内含 0 .0 2M磷酸和 0 .2 %十二烷基硫酸钠 ) ,色谱柱YWG18(4 .6× 15 0mm) ,紫外检测波长为 2 75nm ,柱温 40°C ,流速 1ml/min。结果 :升压灵注射液静注后在兔体内呈二室模型分布 ,其药代动力学参数是Vc=5 3 .81ml/kg ,K12 =0 .2 7/min ,K2 1=0 .13/min ,K10 =0 .6 /min ,T1/ 2 α =0 .76min ,T1/ 2 β =8.2 3min ,α =0 .92min ,β =8.42× 10 -2 /min ,AUC =2 76 .36 μg/L·min ,Cl=32 .0 5ml/min。 结论 :临床上使用升压灵注射液时 ,可短期多次或缓慢静脉滴注给药更多还原

【Abstract】 Objective: To determine pharmacokinetic parameter in vivo of Shengyaling Injection in rabbit. Methods: Using synephrine in Shengyaling Injection as a marker, the determination had been carried out by RP HPLC. The HPLC conditions were methanol: water [55∶45, containing 0.02 Mol/L H 3PO 4 and 0.2% SLS] as mobile phase, chromatograph column of YWG18 (6×150mm), at 275nm of UV detector wavelength, 40 °C column temperature and 1ml/min of mobile rate. Results: After i.v the injection in rabbit, the plasma concentration time data was found to be in accordance with the 2 compartment model. Its pharmacokinetics parameter were V c=53.81ml/kg,K 12 =0.27/min,K 21 =0.13/min,K 10 =0.6/min,T 1/2α =0.76min,T 1/2β = 8.23 min,α=0.92min,β=8.42×10 -2 /min,AUC=276.36μg/L·min,Cl=32.05ml/min. Conclusion: Shengyaling Injection can be used in clinic by short periods for many times or slowly in travenous guttae.更多还原