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RP-HPLC测定洋参二醇皂甙注射液中人参皂甙Rb3的含量
Determination of Ginsenoside Rb3 in Panaxadiol Saponin of American Injection by RP HPLC
【摘要】 目的 :测定洋参二醇皂甙注射液中人参皂甙Rb3 的含量。方法 :HPLC。流动相 :乙腈 -水 -磷酸 (31∶6 9∶0 .2 ) ,检测波和 2 0 3nm。结果 :人参皂甙Rb3 含量测定线性范围 1μg~ 9μg ,加样回收率 99.16 % ,RSD 0 .96 %。 结论 :本法简便、灵敏、准确、可作为该制剂的质量控制方法之一。
【Abstract】 Objective: To determine ginsenoside Rb 3 content in Panaxadiol Saponin of American Injection.Methods: HPLC; mobile phase: acetonitrile water phosphoric acid (31.69∶0.2); detection wavelength at 203nm. Results: The linear range of ginseroside Rb 3 content determination was from 1μg to 9μg. The recovery of the added sample was 99.16%. RSD was 0.96%. Conclusion: The method in simple, sensitive and accurate, and can be used as one of methods for quality control of this injection.
【关键词】 高效液相色谱法;
洋参二醇皂甙注射液;
人参皂甙Rb3;
【Key words】 HPLC; Panaxadiol Saponin of American Injection ginsenoside Rb 3.;
【Key words】 HPLC; Panaxadiol Saponin of American Injection ginsenoside Rb 3.;
- 【文献出处】 中成药 ,CHINESE TRADITIONAL PATENT MEDICINE , 编辑部邮箱 ,2000年05期
- 【分类号】R284.1
- 【被引频次】1
- 【下载频次】75