【摘要】 目的 :观察脂多糖 (LPS)对体外培养的系膜细胞 (GMC)产生NO的影响及这一过程对GMC增殖的影响。方法 :用培养的第一、二代SD大鼠肾小球系膜细胞进行实验 ,分别加入LPS或LPS加白细胞介素 - 1受体拮抗剂 (IL - 1ra) ,2 4h后用经典的Griess方法测定培养上清中亚硝酸盐含量 ,用 [3 H]-TdR掺入率测定GMC的增殖 ,狭缝和Northern杂交测定iNOSmRNA表达。结果 :LPS刺激后 ,GMC出现iNOSmRNA的表达及亚硝酸盐增多 [(0 6 4± 0 2 5 )nmmol/ 10 4 细胞vs (0 12± 0 0 6 )nmmol/ 10 4 细胞 ],GMC增殖 (3735± 1177 9vs 1785± 2 80 6 ) ;LPS加IL - 1ra组 ,iNOSmRNA表达比LPS组减少 40 % ,上清亚硝酸盐含量更高 [(3 2 8± 0 33)nmmol/ 10 4 细胞 ],GMC增殖被抑制 (818± 77 2 7)。结论 :LPS诱导GMCiNOSmRNA表达及亚硝酸盐增多 ,并有GMC增殖。IL - 1ra部分拮抗iNOSmRNA的表达 ,但亚硝酸盐产量更高 ,GMC增殖被抑制更多还原

【Abstract】 AIM: To investigate the effects of Lipopolysaccharide(LPS) and interleukin 1 receptor antagonist(IL-1ra) on mesangial cells proliferation and nitric oxide synthesis. METHODS: Glomerular mesangial cells from SD rats were cultured. The first and second passages of cultured cells were used for the experiment. LPS and LPS plus IL-1ra were added in cell cultures, respectively. By using chemical method the nitrite in supernatants was measured,3H-TdR incorporation was determined to evaluate the GMC proliferation. Northern and slot hybridizations were performed to detect the expression of iNOS mRNA. RESULTS: There were expression of iNOS mRNA, more production of nitrite(0.64±0.25 vs 0.12±0.06 nmol/104 cell) in supernatants and GMC proliferation(3735±1177.9 vs 1785±280.6) in LPS group compared to the control. While compared with LPS group, in LPS+IL-1ra GMC group, expression of iNOS mRNA decreased by 40%, nitrite increased(3.28±0.33 nmol/104 cell), proliferation of GMC decreased (818±77.27). CONCLUSION: LPS could activate the GMC to express iNOS mRNA and produce more nitrite. IL-1ra could partially inhibit the effects of LPS on the expression of iNOS mRNA in GMC, but not nitrite. There is no synchronous correlation between NO production and GMC proliferation.更多还原