【摘要】 从我国内蒙古地区流行的犬细小病毒病病犬的肠溶物中分离提纯犬细小病毒 (CPV)。提取病毒基因组DNA ,并以此DNA为模板 ,采用人工合成的引物进行PCR扩增 ,PCR产物经BamHI、SacI双酶切后 ,克隆于 pUC19质粒的BamHI/SacI位点。重组质粒 pUCVP2经PCR鉴定、限制酶切分析和序列分析 ,结果表明 :获得了犬细小病毒内蒙株 (CPV IM )VP2基因的全长克隆 ,VP2基因全长 1755nt,与国外报道的美国 1株 (CPV N)、美国 2株 (CPV B)和猫全白细胞减少症病毒(FPLV)的核苷酸序列同源性分别为 99.32 %、98.2 9%、98.52 % ,氨基酸序列同源性分别为98.87%、97.0 9%、97.77%。更多还原

【Abstract】 The DNA of Canine Parvovirus Inner Mongolia isolate (CPV IM) was isolated from the enteric lysate of virus infected dog showing enteritis symptoms. The VP2 gene of CPV IM was amplified by PCR using designed and synthesized two primers and cloned into plasmid pUC19 at Bam H I/ Sac I sites. Recombinant plasmid pUCVP2 was identified by PCR and restriction enzymes analysis and nucleotide sequence analysis. Results show that the full length VP2 gene clone of CPV IM was obtained. VP2 gene of CPV IM isolate consists of 1 755 nt, and there is a high homology in nucleotide sequence and amino acid sequence in comparison with CPV N, CPV B and Feline Panleukopenia Virus (FPLV). The rate of nucleotide sequence homology is 99.32%, 98.29% , 98.52%, and the rate of amino acid homology is 98.97%, 97.09%, 97.77%, respectively.更多还原