【摘要】 目的　确诊棘阿米巴角膜炎 ,了解棘阿米巴角膜炎的临床表现和病理特征。方法　 10 %氢氧化钾 (KOH )湿封片镜检、棘阿米巴原虫培养、倒置显微镜观察、病理切片作H .E .、PAS和PAS加亮绿染色检查。结果　角膜刮片经10 %KOH湿封片镜检和原虫培养共诊断 9例棘阿米巴角膜炎 ,其中 8例表现为环型角膜基质炎 ,7例眼球剧烈疼痛。病理切片经上述染色可显示棘阿米巴原虫的包囊和滋养体。结论　环型角膜基质炎和眼球剧烈疼痛是棘阿米巴角膜炎的主要临床特征 ,病理切片经上述染色显示原虫的包囊和滋养体 ,PAS加亮绿染色 ,原虫的滋养体呈深绿色 ,更清晰更多还原

【Abstract】 Objective To investigate the clinal and pathological characteristics of acanthamoeba keratitis.Methods10% potassium hydroxide(KOH) wet mount, Acanthamoeba protozoa culture,observation and identification directly under inverted phase contract microscope,pathological examination by HE,PAS and PAS joining with light green staining were performed.ResultsEight of 9 cases from corneal scrapings diagnosed by wet mount showed corneal ring shaped astromal infiltration and severe ocular pain. Pathological specimens stained with HE,PAS,and PAS and counter staining with light green showed the cysts and trophozoites of Acanthamoeba protozoa .ConclusionThe corneal ring shaped astromal infiltration and severe ocular pain are main characteristics of Acanthamoeba keratitis .The cysts and trophozoites of Acanthamoeba protozoa could be observed by HE.,PAS and PAS and counter staining with light green.更多还原