【摘要】 采用高效液相色谱法测定可可粉和可可壳酊中的可可碱及咖啡因含量 ,固定相为Hyper silODS ,流动相为甲醇 -醋酸 -水 (2 0∶1∶79) ,紫外检测波长 2 75nm。样品中可可碱和咖啡因的回收率分别为 98.6 %± 0 .71%和 97.1%± 0 .80 % ,变异系数为 0 .80和 1.4 9。更多还原

【Abstract】 A high performance liquid chromatography method for determination of theobromine and caffeine in cocoa powder and cacao shell tincture was established Theobromine and caffeine were separated from cacao products by using Hypersil ODS column (125mm × 4mm i.d.) and mobile phase of methyl alcohol acetic acid water (20∶1∶79) with a flow rate of lml/min. The eluates were monitored with DAD detector at 275 nm. The average recoveries of theobromine and caffeine were 98.6%±0.71% and 97.1%±0.80% respectively. The RSD of theobromine and caffeine were 0.80 and 1.49 respectively.更多还原