【摘要】 目的　研究白桦三萜类物质 (triterpenesofbetulaplatyphyllasuk .TBP)抗黑色素瘤B16、S180肉瘤及其诱导细胞调亡作用和对细胞周期的影响。方法　建立小鼠体内荷黑色素瘤B16和腹水型S180肉瘤模型 ,测定TBP的抑瘤率和生命延长率。用形态学检测方法 (Giemsa染色法 )和流式细胞光度术检测TBP诱导的细胞调亡和对细胞周期的影响。结果　TBP具有明显的抗肿瘤作用 ,1 2 g·kg-1TBP对黑色素瘤B16的抑瘤率为 5 1 40 % ,对荷S180肉瘤小鼠生命延长率为 41 0 4%。可诱导B16和S180肿瘤发生细胞调亡 ,G0 /G1期细胞比例增加 ,S期细胞比例下降。结论　TBP可明显抑制黑色素瘤B16和S180肉瘤生长 ,其机制与诱导细胞调亡和阻断细胞生长于G0 /G1期有关。更多还原

【Abstract】 AIM To study antitumor activity of Triterpenes isolated from Betula Platyphylla (TBP) on melanoma B16 and sarcoma 180 and effect of TBP on apoptosis and cell cycle. METHODS Antitumor activity of TBP was determinded by the mice with transplantable tumor (melanoma B16 and sarcoma 180). Apoptosis and cell cycle were analysed by flow cytometry (FCM). RESULTS TBP showed significant antitumor activity effect. Inhibitary rate (IR) of 1 20 g·kg -1 TBP on melanoma B16 was 51 40%,and increase in life span ( ILS ) on sarcoma 180 was 41 0%. TBP induced apoptosis and enhanced the percentage of cells in G 0/G 1 phase and decreased these in S phase in vivo . CONCLUSION TBP shows potent antitumor effect on melanoma B16 and ascitic sarcoma 180. Inducing apoptosis and arresting tumor cell in G 0/G 1 phase is one of antitumor mechanism of TBP.更多还原