【摘要】 目的建立一种人外周血γδ+ T细胞体外选择性扩增的模式 :即在IL 2的作用下 ,不需要γδ +TCR特异性配体(或抗原)的刺激 ,可使一类个体体外培养的外周血单个核细胞(PBMC)中的γδ+ T细胞发生优先增殖 ,逐渐成为培养细胞的主要群体。方法将10个γδ+ TCR的个体的PBMC分别在含3×105U/LIL 2培养液中培养1～2wk后 ,其中的γδ+ T细胞的比率可达38 %～78 %。结果对3个这样的个体的扩增细胞做RT PCR分析表明 ,扩增出的γδ +T细胞表达TCRVδ2 基因片段。这种γδ +T细胞体外优先扩增的性质类似于记忆性αβT细胞的体外生长特征 ,可能反映了γδ +T细胞在体内的致敏状态。结论该现象将有利于进一步了解γδ+ T细胞在免疫系统中的地位和进化上的意义。更多还原

【Abstract】 Aim To establish one pattern of human peripheral γδ+T cells growth in vitro. Methods Without the stimulation of specific γδ+TCR ligands, the γδ+T cells in the cultured peripheral blood mononuclear cells(PBMC) of 10 selected donors preferentially proliferated in a dose dependent manner to IL 2. After 6 14 days of culture in the medium containing 3×105U/L IL 2, the percentages of γδ+T cells rose to 38% 78%with the increase of cumulative cell numbers. Results RT PCR analysis of the expanded γδ+T cells from 3 typical donors showed that they expressed TCR Vδ2 gene fragments. This kind of γδ+T cell expansion was similar to the memory αβT cell growth in vitro, which might represent the primed state of γδ+T cells in vitro. Conclusion This phenomenon will be beneficial for the understanding of γδ+T cells positions in immune system and significance in evolution.更多还原