【摘要】 目的　预测肺癌手术切除的效果和局部复发情况。方法　应用聚合酶链—单链构象多态分析 (PCR SSCP)方法 ,检测肺癌手术切除标本的无癌支气管切缘和病灶癌组织 p5 3基因突变的情况。 结果　 10例支气管切缘有 p5 3基因突变 ,阳性率2 5 .0 %( 10 /40 )。 2 1例病灶癌组织有 p5 3基因突变 ,阳性率 5 2 .5 %( 2 1/40 )。术后全部病例随访 3～ 14个月。在 10例支气管切缘p5 3阳性者中 ,有 4例发生支气管残端癌复发 (复发率 40 .0 %) ,而在 3 0例阴性者中 ,无 1例发生残端癌复发。 2 1例病灶癌组织 p5 3阳性者中 ,有 12例发生癌复发转移 (复发率 5 7.1%) ,19例阴性者中 ,4例发现癌复发转移 (复发率 2 1.1%)。结论　分子学诊断早于临床诊断 3～ 6个月。检测支气管切缘p5 3基因突变 ,能早期预测部分肺癌的术后支气管残端癌复发。病灶癌组织 p5 3阳性者 ,术后癌复发转移率高。更多还原

【Abstract】 Objective To invesgate the clinical significane of p53 gene mutation at lung cancer resection margin.Methods Cancer tissues and non cancer brochus margin from 40 patients with lung cancer resection were studied by PCR SSCP technique. Results p53 gene mutation showed in 25.0%(10/40) bronchus margins and 52.5%(21/40) cancer tissues.Follow up for 3～14 months,bronchus residual cancer occured in 40.0%(4/10) of the patients with p53 mutation at brochus margin,while no recurrence was seen in the patients without p53 mutation.The recurrence and metastasis rate was 57.1% in patients whose cancer tissue was p53 mutation positive,21.1% in that of p53 mutation negative.Conclusion Detection of p53 gene mutation can provide a prediction for recurrence of postoperation bronchus residual cancer.更多还原