【摘要】 目的　进一步研究ＲＡ５３８ 对人结肠癌细胞系的生物学效应，选用介导ＲＡ５３８ 重组体腺病毒，观察其对人结肠癌细胞系ＨＣＴ 的作用。方法　通过重组体腺病毒将ＲＡ５３８ 基因转导到人结肠癌细胞系ＨＣＴ 中，观察转染ＲＡ５３８ 基因后的肿瘤细胞生长情况。经流式细胞仪、ＤＮＡ 片段化分析及ＴＵＮＥＬ 检测分析抑瘤机制。结果　由重组体腺病毒ＡｄＲＡ５３８ 介导基因ＲＡ５３８ 转导在ＨＣＴ 获得较高的转导效率， ＭＯＩ 为１００ 时可使８０ ％ 细胞转染，并显示对肿瘤细胞生长有明显的抑制作用，抑制率为７４ ％ 。裸鼠皮下注射ＲＡ５３８ 处理的ＨＣＴ 细胞肿瘤形成能力为２０ ％ 。ＤＮＡ 片段化分析、ＴＵＮＥＬ 和流式细胞仪检测，结果证明ＲＡ５３８诱导肿瘤细胞发生凋亡。Ｗｅｓｔｅｒｎ ｂｌｏｔ 分析表明，ＲＡ５３８ 具有明显下调ｃｍ ｙｃ 基因表达的作用。结论　ＲＡ５３８ 对人结肠肿瘤细胞有抑制生长和诱导凋亡的作用，可以作为结肠肿瘤基因治疗的１ 种新的治疗基因。更多还原

【Abstract】 Objective To study the antitumor effects of RA538 on human colon carcinoma cell line HCT.Methods Gene transfer efficiency was determinated,by the growth rate of HCT cells after infection with Ad RA538,the analysis of flow cytometry and DNA fragmentation,TUNEL technique,western blot analysis.Results Transfection of RA538 gene into human colon carcinoma cell line(HCT) by recombined adenovirus showed an obvious suppression to tumor growth,the tumorigenicity of HCT cells infected with Ad RA538 followed by into nude mice was 20%.Result of DNA fragmentation analysis,TUNEL and FCM assays demostrated that the tumor cell can be induced to apoptosis by Ad RA538.Western blot analysis showed that RA538 has a function of downregulating c myc gene expression.Conclusion RA538 can inhibit the growth of HCT cells and induce the cell apoptosis.It could be a new therapeutic gene in gene therapy of human colon carcinoma.更多还原