【摘要】 目的　观察静脉注射用免疫球蛋白与免疫抑制剂偶联物对单核—巨噬细胞的杀伤作用 ,为其临床治疗ITP奠定实验基础。方法　分别用间接和直接交联法将IVIG与MTX制备成偶联物 ,用间接免疫荧光法检测偶联物Fc段结合活性 ,以Fc受体阳性的小鼠巨噬细胞和人单核细胞白血病细胞株U937为靶细胞 ,用MTT法测定偶联物的杀伤活性。结果　偶联物对巨噬细胞的杀伤作用明显大于游离MTX ;偶联物对Fc受体阳性细胞的杀伤作用明显大于Fc受体阴性细胞。间接偶联物IVIG HSA MTX杀伤作用明显高于直接偶联物IVIG MTX。结论　MTX抗体偶联物在体外对单核—巨噬细胞显示了相对特异的杀伤活性更多还原

【Abstract】 Objective To observe the blocking and killing effect of MTX IVIG conjugate on mononuclear macrophage and lay an experimental foundation of clinical application of it in treating idiopathic thrombocytopenic purpura (ITP).Methods MTX IVIG conjugate was prepared by indirect and direct cross linking,and the binding ability of it to Fc fragment was detecteded by indirect immunofluorescence.The killing activity of the conjugate was detected by MTT method using murine macrophage with Fc receptor and strain U937 of human monocytic leukemia cell as targets.Results Compared with free MTX,the conjugate showed stronger killing effect on macrophage,and the effect on the Fc receptor positive cells was significantly stronger than that on Fc receptor negative cells.However,the killing effect of indirect conjugate IVIG HAS MTX was significantly stronger than that of direct conjugate IVIG MTX.Conclusion IVIG MTX conjugate shows relatively specific killing effect in vitro on mononuclear macrophage.更多还原