【摘要】 以大孔纤维素滤纸为基质 ,通过碱处理、环氧活化、偶联亚氨基二乙酸二钠、固定化Cu2 +,装柱后制得固定化金属螯合亲和膜色谱柱。对Cu/Zn SOD粗品进行了纯化研究 ,将其比活从 645U/mL提高到 6882U/mL ,纯化倍数为 1 0 7,蛋白回收率为 92 3% ,活性回收率达 985%。设计了一种解决金属离子泄露问题的方案 ,将Cu2 +泄露量降至 86μg/L。更多还原

【Abstract】 Immobilized metal chelated affinity membranes were prepared using macropore cellulose filter paper as matrix The matrix was treated with alkaline, activated with epichlorohydrin and coupled with iminodiacetate sodium, and then Cu\+\{2+\} was immobilized Cu/Zn superoxide dismutase(SOD) was purified with prepared affinity membranes The special activity of the purified Cu/Zn SOD was increased 10 7 times from 645 U/mL to \{6 882\} \{U/mL \} The protein recovery and the activity recovery were 92 3% and 985%, respectively A new method to prevent the leaking of metal ion was developed and Cu\+\{2+\} concentration in the effluent was lowered to 86 μg/L更多还原