【摘要】 对青春双歧杆菌在胡萝卜汁复合培养基中生长所需的营养物质作了进一步的调查 ,考察了番茄汁、黄豆芽汁、葡萄糖、氯化钠等的添加对双歧杆菌的促生情况。采用正交试验法研究了青春双歧杆菌在复合蔬菜汁中的发酵工艺参数 ,考察了该菌在 5L发酵罐中的发酵曲线 ,发酵最高活菌数约为 5.1 0× 1 0 1 1 个 / ml。最终确定了 5L发酵罐中的培养时间、温度、p H值、接种量等最佳工艺参数。更多还原

【Abstract】 Some kinds of nutritions for promoting growth of Bifidobacterium adolecsentis in mixed medium of vegetable juice was investigated and the fermentative optimum conditions was also determined according to orthogonal test L 9(3 3) about temperature ,pH value and concentraton of starter. It was found that the glucose,sodium chloride, tomato juice and soybean bud juice was capable of promoting gowth of Bifidobacterum while at the concentration of 2%, 0.1%, 25% and 12% respectively ,and that the fermentative temperature had the obvious effect on growth of Bifidobacterum . The number of living cell rose up to the level of 5.10×10 11 cfu/ml after anaero cultivating according to the fermentative optimum conditions based on conclusion of the study.更多还原