【摘要】 在大鼠肢体缺血模型上观察了质粒pcDNA3对缺血骨骼肌肌浆网 (SR)Ca2 + 转运的影响。结果显示 ,骨骼肌缺血时SRCa2 + 转运 (Ca2 + 摄入与释放 )较非缺血肌肉增强 ,而质粒pcDNA3与SR上DNA结合蛋白结合之后 ,可进一步增强缺血骨骼肌SRCa2 + 摄入 (P <0 0 1)及释放速率 (P <0 0 5 )。提示质粒DNA对正常及缺血大鼠骨骼肌的SRCa2 + 转运能力均有影响 ,其临床病理生理意义值得进一步研究。更多还原

【Abstract】 The effects of plasmid pcDNA3 on Ca 2+ transport of sarcoplasmic reticulum (SR) in ischemic skeletal muscle was investigated. The results show that Ca 2+ transport (including Ca 2+ uptake and Ca 2+ release) rate of SR in ischemic skeletal muscle was markedly increased compared with that in non ischemic muscle (P<0 01 or P<0 05). After plasmid pcDNA3 bound to the DNA binding proteins of SR, Ca 2+ transport of SR was further increased. The results suggest that the effect of plasmid DNA on the ability of Ca 2+ transport in SR of ischemic skeletal muscle is the same as is observed in normal skeletal muscle. The pathophysiological significance of the present finding deserves further exploration.更多还原