【摘要】 目的　研究肝纤维化时Ⅳ型胶原酶（ＭＭＰ２）和金属蛋白酶组织抑制因子１（ＴＩＭＰ１） 在肝组织与贮脂细胞（ＦＳＣ） 中的表达，探讨其对肝纤维化的作用。方法　分别从正常和ＣＣｌ４ 肝纤维化大鼠的肝组织及ＦＳＣ中提取ＲＮＡ，应用逆转录聚合酶链反应（ＲＴＰＣＲ）方法检测ＭＭＰ２ 与ＴＩＭＰ１ ｍＲＮＡ 水平。结果　正常肝脏ＦＳＣ不表达ＭＭＰ２ ，仅肝纤维化时才转录；肝组织中无论是正常或肝纤维化时均存在ＭＭＰ２ ｍＲＮＡ，并在肝纤维化时表达增加，但无显著性差异（ Ｐ＞０ ．０５） 。ＴＩＭＰ１ 在正常与肝纤维大鼠的肝组织或ＦＳＣ中均表达，肝纤维化时肝组织和ＦＳＣ的ＴＩＭＰ１ ｍ ＲＮＡ 水平与正常相比显著升高（ Ｐ＜０ ．０５，Ｐ＜ ０．０２） 。结论　ＦＳＣ 表达ＭＭＰ２对肝纤维化发生具有重要意义，而ＴＩＭＰ１ 表达增加可能是导致肝纤维化过程中胶原降解减少的主要原因之一。更多还原

【Abstract】 Objective To investigate the role of matrix metalloproteinase 2 (MMP 2) and tissue inhibitor of metalloproteinases 1(TIMP 1) during hepatic fibrogenesis. Method Semiquantitive evaluation of MMP 1 and TIMP 1 mRNA were determined by RT PCR in liver tissue and fat storing cells (FSC) respectively.Results MMP 2 was undetectable in FSC isolated from normal rat liver, but it was expressed in FSC isolated from fibrotic rat liver. Both normal and fibrotic liver tissue expressed MMP 2. There was no significant difference between them( P >0.05), although the MMP 2 mRNA level was increased in fibrotic liver tissue. TIMP 1 mRNA was detected either in liver tissue or FSC, whatever it was from normal or fibrotic liver, but it was increased remarkably in both FSC and tissue obtained from fibrotic liver( P <0.05, P <0.02) respectively. Conclusion Expression of MMP 2 from FSC plays an important role in hepatic fibrogenesis, increases of TIMP 1 expression is probably a major factor for the reduction of collagen degradation.(Shanghai Med J, 2000,23∶73 75)更多还原