【摘要】 利用固相多肽合成中的Boc途径合成了人胰岛素原C肽 ,经TSK柱层析一步纯化 ,产物达HPLC及毛细管电泳均一 ,蛋白质序列分析和质谱分析符合理论值 ,总回收率高达 41 %。为了提高寡肽抗体的专一性 ,我们将丙烯酰化的C肽经催化聚合形成了以聚丙酰为骨架 ,C肽为侧链的聚合体 ,分子量约为 2 5kD。免疫新西兰大白兔 1月 ,获得专一性抗体。用酶联免疫吸附法测得抗血清滴度达 2 .5× 1 0 4 ,与BSA无交叉反应。聚丙酰C肽抗原是制备C肽抗体的一种新的尝试 ,而且也可能成为新的多肽工程疫苗之一 ,为其他传染性疾病多肽疫苗的研究提供新的途径更多还原

【Abstract】 A HPLC and CE pure human proinsulin C peptide was sy nthesized by solid phase method and TSK column purification. Its amino acid seq uence and MS were consistent with theoretical values. In comparison with the for mly reported chemical synthesis of C peptide, this method has the advantage of simplicity and higher overall yield (41%). To improve the immunogenicity and spe cificity of oligopeptide antibody, the acrylyl C peptides were transformed int o a polymer; the product had a poly propionyl core matrix with C peptide bra nches. This treatment gave a macromolecule with a M r about 25 kD. By using the polymer to immunize New Zealand rabbits for 30 days, specific antiserum was obtained with titer of 2.5×10 4 (by ELISA), which did not cross react with BS A. Thus, the poly propionyl peptide system provided a new approach for prepari ng synthetic peptide antibody and therefore is promising for the preparation of synthetic peptide based vaccine.更多还原