【摘要】 通过贴壁的方法分离胚肝、脐带血、造血动员后的外周血及正常人外周血中的树突状细胞 (DC )的前体细胞 ,体外采用细胞因子诱导 ,并对其进行形态学观察和细胞表型分析 ;同时比较了不同来源DC的增殖、分泌IL 12的能力及激发同种异体T细胞和脐带血幼稚型T细胞 (naiveTcell)的作用。实验结果表明 ,经过造血动员后的外周血细胞产生的DC数量和纯度均较高 ,而且能经体外细胞因子诱导为功能性DC ,是较理想的DC来源更多还原

【Abstract】 The adherent precursors of dendritic cells (DC) isolated from fetal liver,cord blood,mobilized or nonmobilized peripheral blood were induced with cytokines to DC in vitro The growth of DC from different sources,and their ability to secrete IL12 and their potentials to stimulate the proliferation of naive T cells from cord blood were observed It was found that DC precursors from peripheral blood after mobilization were a good source of DC,and thus produced DC showed a high quantity of production and higher purity,and to be fully functional更多还原