【摘要】 目的 :进一步研究氯丙烯的中毒机理 ,探讨氯丙烯对神经细胞内钙稳态与能量合成的影响。方法 :用 Fura- 2 /AM、电子探针 X线微区分析、ATP生物发光分析等方法测定氯丙烯染毒后鸡胚脑神经细胞内游离 Ca2 +、钙百分含量及 ATP、ADP、AMP含量的改变。 结果 :随着氯丙烯浓度的增加 ,细胞内游离Ca2 + 浓度、钙百分含量明显增加 ( P<0 .0 1 ) ;细胞内 ATP、ADP、AMP含量明显减低 ,细胞外 ADP、AMP含量明显增高 ( P<0 .0 1 )。结论 :氯丙烯可引起神经细胞内 Ca增加和 ATP合成减少 ,氯丙烯引起的细胞内 Ca稳态失调可能与能量合成减少有关。更多还原

【Abstract】 Objective: To research the poisoning mechanisms of allyl chloride and probe into the action of allyl chloride on calcium homeostasis and energy synthesis in brain cells.Methods: Contents of Ca, ATP, ADP, AMP were studied in allyl chloride induced chicken embryo brain cells with the methods of fluorescence molecular probe Fura 2/AM, electron probe X ray microanalysis(EXMA)and ATP bioluminescence analysis. Results: With the increase of allyl chloride, the contents of Ca 2+ increased significantly (P<0.01), while the contents of ATP, ADP, AMP markedly decreased(P<0.01).Conclusion: The results suggested that allyl chloride can increase the concents of calicium and decrease energy synthese in nerve cells. Inhibition of energy synthesis in duced by allyl chloride in nerve cells might be one of the mechanisms of calcium unhomeostasis.更多还原